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Differential estrogenic activity of Pueraria mirifica leaves determined by yeast estrogen screen harbored human estrogen receptor -α and -β

Proceeding from jhptump / 2018-02-10 12:22:21
Oleh : Binar Asrining Dhiani. Jutarmas Jungsukcharoen, Chuenchit Boonchird, Wichai Cherdshewasart , Universitas Muhammadiyah Purwokerto (
Dibuat : 2013-03-25, dengan 1 file

Keyword : Pueraria mirifica leaves; Estrogenic Activity; YES method; hER; SNQ2

Leaves of P. mirifica, which are available abundant throughout year, were determined its estrogenic activity following the extensive studies of its tuberous part [1-6]. Crude extracts of monthly harvested P. mirifica leaves from cultivar PM III, PM IV and PM V were subjected to estrogenic activity determination using Yeast Estrogen Screen (YES) method involving human estrogen receptor-(hER-)α and hER-β. Tuberous extracts were also evaluated as comparison. The yeast strains used in this study were wild type [7] and SNQ2 deletion (snq2Δ) strain. The YES systems were confirmed its sensitivity using pure phytoestrogen compounds prior to determination of the leaves extracts. The results (IC50 as parameter) exhibited that P. mirifica leaves extracts of all cultivars in all harvested months had higher estrogenic activity determined by YES system harbored hERβ than hERα. Estrogenic activity was significantly higher (p≤0.05) when SNQ2 deletion was employed. Furthermore, estrogenic activity of P. mirifica leaves extracts was slightly lower than its tuberous extracts.

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  • a Fac.of Pharmacy, Muhammadiyah University of Purwokerto, Jalan Raya Dukuhwaluh, Purwokerto Indonesia

    b Dept.of Biology, Fac.of Science, Chulalongkorn University, Pathumwan Road, Bangkok, Thailand

    c Dept.of Biotechnology, Fac.of Science, Mahidol University,272 Rama 6 Road, Bangkok, Thailand, Editor: