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FUNDAMENTAL ASPECTS OF SPERM CRYOPRESERVATION

Proceeding from JHPTUMP / 2017-03-08 19:49:15
Oleh : Quoc Anh, N., Catt, S., Temple-Smith, P., Chen, P., Pangestu, M., Monash University
Dibuat : 2017-01-12, dengan 3 file

Keyword : sperm cryopreservation, DNA, HSA.

Objective: to test the effects of in conventional slow cooling of mouse and human sperm and to examine the quality of mouse and human sperm after vitrification with and without cryoprotectants. Design: Experimental study. Samples: Fifteen male Ba1b/c mice at 8 weeks were collected. Six healthy men participated in this study. Main outcome measure(s): post-thaw motility rates of mouse sperm after vitrification with and without cryoprotectants, post-thaw motility rates of human sperm after vitrification with and without cryoprotectants, post-thaw motility rates of human sperm after slow cooling with and without Human Serum Albumin (HSA), DNA integrity changes of human sperm during vitrification. Result(s): Mouse: no motile sperm was detected after thawing in both cases: with and without cryoprotectants. Human:, low motiity but high DNA integrity was detected after vitrification in minimal volumes without cryoprotectants. On the other hand, vitrification with cryoprotectants resulted in no sperm motility after thawing. HSA had an effect on slow-cooled human sperm and its effect varied between the samples tested. Conclusion(s): Vitrification in minimal volumes without cryoprotectants can potentially become a mainstream application in sperm cryopreservation, especially in cases where sperm volumes or sperm numbers are very low, such as from testicular biopsy samples. HSA may offer protection during sperm cryopreservation by slow cooling but a larger sample size is required to define its use.

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Organisasi Monash University
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